ThewhitelightlasersourceoftheLeicaTCSSP8Xperfectlymatchesthewavelengthofanyfluorophore.Uptoeightexcitationlinescanbeused–simultaneously.
Bytuningbothexcitationanddetection,completeexcitationemissionspectracanbeacquired.
Withthisspectralinformation,anydyecanbeoptimallyexcitedwithminimumcross-excitationandspecimendamage.
Inaddition,thepulsedWhiteLightLaser(WLL)suppliesexcitationlightforfluorescencelifetimemeasurements.
FLIMprovidesinformationaboutthedirectenvironmentofamolecule,likelocalpHorbindingtoanothermolecule.
Completespectralfreedom
Thechoiceoffluorescentlabelsisnotlimitedanylongerbythehardware.TheresearcherselectsthedyesaccordingtotheiravailABIlityorthescientificneeds.Withuptofivespectraldetectionchannels,theLeicaTCSSP8Xiscompletelytunableandfullyadaptstothespecimen.
Fullspectralcharacterizationofimages
Newdyesorfluorescentproteinscanbediscoveredandcharacterized.Atwo-dimensionalexcitation-emissionspectrumcharacterizesthespectralpropertiesofanyfluorescentsample.Fluorophoreswithidenticalemissionorexcitationspectraarediscriminated.Thisisessentialformulti-colorfluorescenceexperiments.
ZerobackgroundbyLightGate
LightGateisaclever,filter-freemethodtoremoveunwantedsignalfromtheimagedata.Anadjustabletimegateswitchesoffthedatacollectionduringthewhitelightlaserpulse.Additionally,non-wantedfluorescencecanberemovedbyadjustingthetimegate.Thiswaythehighestimagecontrastwithoutanyreflectioncontributionisobtained.
FreeselectionofFLIMexcitationwavelength
TheWhiteLightLaserisanappropriateexcitationsourceforfluorescencelifetimemeasurements.ItscontinuoustunabilityallowsperformingFLIMmeasurementatanyexcitationwavelength.Lifetimeoforangeandreddyescannowbeobtained.Theoptimallaserrepetitionrateisselectedviaanintegratedpulsepicker.
目前拆分为三家公司：徕卡相机股份公司、徕卡地理系统股份公司和徕卡微系统有限公司，分别生产照相机、地质勘测设备和显微镜。“徕卡”品牌由徕卡微系统股份公司持有，并授权另两家公司使用。徕卡相机最初问世于1913年，是世界上最早35mm的照相机。
819一次性窄刀片为80mm长x8mm高x0.25mm厚。
由不锈钢制成，刀片超细而耐用，能够用于徕卡切片机和冰冻切片机系列，以及用于常规的冰冻切片机和切片机模型。
独特的刀片涂层工艺对常规组织学中切片拉伸与切割条带质量的改进效果显而易见。
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